HPLC has been used for many years and the technique is widely found in pharmacopoeia monographs. The main difference between HPLC and UPLC is the systems’ pressure. UPLC was created along with the development of submicron (<2 microns) LC columns to increase the efficiency of liquid chromatography separations.
Increasing the surface area of the column allows for shorter columns to be used, which reduces the separation time and run times without any reduction of the resolution between compounds, often with improved resolution.
Both techniques are widely used for organic and inorganic materials. They are especially useful for determining purity, monitoring reactions and production processes and characterising complex materials.
Our Chromatography Laboratory has a wide range of detectors available to accommodate the needs of our clients, including:
- UV (Single wavelength)
- Photo Diode Array (PDA)
- Fluorescence (FL)
- Refractive Index (RI)
- Evaporative Light Scattering Detector (ELSD)
- Mass Spectrometry (MS)
We also have a Hitachi LA8080 High-Speed Amino Acid Analyser, which is dedicated to Ninhydrin- Positive Substances (NPS) testing, complying with Ph.Eur 2.2.56 as designated in a number of amino acid monographs. This instrument is also suitable for testing related substances in amino acids, according to the JP monograph. For more details, click here
