For over 40 years, Butterworth Laboratories has provided independent, contract analytical services to the global pharmaceutical and related industries.
How Butterworth uses HPLC to accurately identify free amino acids in biological samples
8 October 2024
High-performance liquid chromatography can highlight the presence of 16 free amino acids in a sample better than its thin-layer chromatography counterpart.
For decades, the determination of ninhydrin-positive substances (NPS) in amino acids relied on thin-layer chromatography (TLC). However, since 2013, high-performance liquid chromatography (HPLC) has progressively replaced TLC in the European Pharmacopoeia due to its superior accuracy and efficiency.
At Butterworth Laboratories, we have adopted HPLC techniques to verify the presence of 16 free amino acids in biological samples, delivering precise and reliable results for our clients.
Our latest white paper delves into this methodology and outlines how our verification process ensures compliance with pharmacopeial standards.
To read the full white paper, click HERE